Lesley Maurice, Bilung and Chandrika, Murugaiah and Zainon, Mohd Noor and Maimunah, Mustakim and Jinap, Selamat and Son, Radu (2009) Meat species identification and Halal authentication analysis using mitochondrial DNA. Meat Science, 83 (1). pp. 57-61. ISSN 0309-1740
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Abstract
A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identification and Halal authentication. PCR products of 359-bp were successfully obtained from the cyt b gene of these six meats. AluI, BsaJI, RsaI, MseI, and BstUI enzymes were identified as potential restriction endonucleases to differentiate the meats. The genetic differences within the cyt b gene among the meat were successfully confirmed by PCR-RFLP. A reliable typing scheme of species which revealed the genetic differences among the species was developed
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Cyt b gene, Mitochondrial DNA, Halal authentication, Forensic science, Meat, PCR-restriction fragment length, polymorphism (RFLP), Species identification, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak. |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Gani |
| Date Deposited: | 10 Aug 2020 05:07 |
| Last Modified: | 20 Jan 2022 02:37 |
| URI: | http://ir.unimas.my/id/eprint/31071 |
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