Nurhaliza, Mohamad Shahidin (2010) Genetic diversity among Jatropha Curcas L. detected by random amplified polymorphic DNA (RAPD) markers. [Final Year Project Report] (Unpublished)
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Genetic diversity among Jatropha Curcas L. detected by random amplified polymorphic DNA (RAPD) markers (fulltext).pdf Restricted to Registered users only Download (9MB) |
Abstract
Jatropha curcas L. (Euphorbiaceae), the oil bearing species is native to Central America having significant economic importance due to its promising potential in biodiesel production. Analysis of genetic diversity of Jatropha curcas L. has become vital due to lack of molecular work done for this species and also due to limited information available for genetic improvement of this crop. Hence, this study was undertaken with an aim to access the extent of genetic variability and genetic relationship among different Jatropha curcas L. accessions from Peninsular Malaysia, Sarawak and Kalimantan. Seven accessions of Jatropha curcas L. were analyzed using Random Amplified Polymorphic DNA (RAPD) marker techniques based on polymerase chain reaction (PCR). Genomic DNA from seven accessions of Jatropha curcas L. was successfully extracted. From the results obtained, the optimum annealing temperature was 33.4°C while the optimum DNA concentration was 20 ng. The optimum annealing temperature and optimum DNA concentration was obtained after several attempts. Optimization of MgCl2 concentration was failed due to failure in amplification. PCR amplification was failed because of failure in optimization of PCR parameters. Genetic variability and genetic relationships between seven accessions of Jatropha curcas L. were not obtained. However, this study could provide preliminary molecular data for the next attempt of genetic diversity study of Jatropha curcas L.
| Item Type: | Final Year Project Report |
|---|---|
| Additional Information: | Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2010. |
| Uncontrolled Keywords: | Jatropha curcas L., Accessions, Genetic diversity, Genetic Relationship, RAPD, PCR, Genomic DNA, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak |
| Subjects: | S Agriculture > SB Plant culture |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Unai |
| Date Deposited: | 17 Jul 2020 02:12 |
| Last Modified: | 08 Jan 2024 08:13 |
| URI: | http://ir.unimas.my/id/eprint/30505 |
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