Kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (Alcyonaceae)

Diana Kertini, Binti Monir (2009) Kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (Alcyonaceae). Masters thesis, Universiti Malaysia Sarawak (UNIMAS).

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Abstract

Isolation and characterization of secondary metabolites and biological activity screening on five species of soft corals Sinularia brassica, S. frondosa, Sarcophyton glaucum, S. trocheliopherum and Cladiella sp. have been performed, Three compounds of indole derivative have been purified and characterized from Sinularia frondosa. The compounds have been identified as N-methyl-3,4,5- tribromoindole, N-methyl-3, 5, 6-tribromoindole and 3,5, 6-tribromoindole. N-methyl3, 4, 5-tribromoindole and N-methyl -3, 5, 6-tribromoindole have been isolated by extensive column chromatography techniques using C6H14 CH2Cl2 (hexane dichloromethane) (1 . 1) and preparative thin layer chromatography using C6H14 Et20 (hexane : diethyl ether) (4 1). 3,5,6- Tribromoindole was purled by column chromatography using CH2C12. N-methyl -3, 4, 5-Tribromoindole and N-methyl-3, 5, 6- tribromoindole were obtained as a white crystalline needles with melting point of 116 - 118 °C and 160 - 162 °C, respectively. 3,5, 6-tribromoindole was obtained as a pale brown crystalline needles with melting point of 106 - 108 °C. The structures of all compounds were established based on various spectroscopic techniques such as infrared, nuclear magnetic resonans ('H- AMR, 13C-NMRO and mass spectrometry. ýToxicity, antifungal and antibacterial screening have been performed on all the five species of soft corals studied' sults showed that all the partition of the soft corals were toxic to the brine shrimps Artemia sauna with LC5o value between 1 - 70 pg/mL. Dichloromethane partition of Cladiella sp. and Sarcoph vton trochehopherum, hexane partition of Sarcophvton glaucum and Sarcophvton trochehopherum and ethyl acetate partition of Sarcophvton glaucum and Sarcophvton trochehopherum and ethyl acetate partition of Sarcophvton glaucum and Sarcophvton trocheliopherum gave strong toxicity against brine shrimp Artemia V sauna with LC5o in a range of 1 - 3 ug/mL. Antifungal screening showed that all the partition obtained from the five species of soft coral studied inhibit Asp piker and Fusarium sp. with inhibition zone of 15.29 - 23.56 % and 15.91- 24.92 respectively at concentration of 80 ppm. Ethyl acetate partition from Sinularia frondosa showed strongest inhibition (23.56 %) towards Asperyillus or n while dicholomethane partition from Sinularia brassica showed strongest inhibiton (24.92 %) towards Fusarium sp.. Antibacterial screening showed that all the partition from the five species of soft corals studied exhibited antibacterial activity at least towards five species of bacteria out of 15 species of bacteria tested at a concentration of 2.5 mg/disk.

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