Comparative Study on Cryptosporidium and Cyclospora Oocysts using Modified Ziehl-Neelsen at Recreational and Environmental Water in Sarawak

Nur Emyliana, Yunos (2017) Comparative Study on Cryptosporidium and Cyclospora Oocysts using Modified Ziehl-Neelsen at Recreational and Environmental Water in Sarawak. [Final Year Project Report] (Unpublished)

PDF (Please get the password by email to repository@unimas.my , or call ext: 082-583914/3973/3933) Nur Emyliana.pdf Restricted to Registered users only Download (15MB)

Abstract

Cryptosporidium species are waterborne parasites that can cause gastrointestinal disease with the manifestation of prolonged and persistent diarrhoea when ingested. Cryptosporidium is introduced into water via point or nonpoint pollution sources. The purpose of this study was to compare the occurrence of Cryptosporidium and Cvclospora oocysts in both recreational and environmental water in Sarawak. Thirty-six water samples (n=36) were collected from recreational areas which were Ranchan Recreational Park (n=6); Universiti Malaysia Sarawak (UNIMAS) Lakes (n=6); and from the environment at Sungai Sarawak Kanan (n=12); Sungai Sarawak Kiri (n=12). The physico-chemical parameters were conducted in situ at all sampling sites. The waterborne parasites then underwent flocculation and sedimentation using ferric sulphate solution followed by sucrose flotation to float the oocysts. The waterborne parasites present were detected by staining with modified Ziehl-Neelsen technique and examined under a compound microscope. Altogether, 18 out of 36 samples were positive for Cryptosporidium while 4 out of 36 samples were positive for Cyclospora. Analysis of waterborne parasites occurrence in both recreational and environmental water was done by using SPSS version 20.0 software. The results of the study indicated that there was a weak correlation between Cryptosporidium and Cyclospora oocyst concentration with the physico-chemical parameters. The distribution of Cryptosporidium and Cyclospora oocysts is same with significant value of 0.721 and 0.333 in both recreational and environmental water. The study may provide database for further control of water quality contributes to prevent of cryptosporidiosis and cyclosporiasis outbreaks.

Actions (For repository members only: login required)

View Item