Induction of Callus and Suspension Culture of Aquilaria microcarpa Baill.

Noor Izzieana, Binti Ibrahim (2013) Induction of Callus and Suspension Culture of Aquilaria microcarpa Baill. [Final Year Project Report] (Unpublished)

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Abstract

Aquilaria microcarpa is a perennial woody plant belongs to Thymeleaceae family. Aquilaria trees produced high value of non-timber products called agarwood. Agarwood are commonly used as incense, fragrance and medicine. This compound form from the respond of the woods towards fungus attack as its defense mechanism. Essential oil of agarwood can be extracted from the callus. This study has been done to improve the growth of callus through suspension culture. This is because; the better growth of callus could produce more extraction of gaharu oil. Callus of A. microcarpa were established on MS medium supplemented with 2.0 mg/L of 2,4-Dichlorophenoxyacetic acid (2,4-D) plus 0.5 mg/L of 6-Benzylaminopurine (BAP). The friable calli obtained from the establishment were used in suspension culture. In suspension culture, the amount of sucrose which is 20, 30, 40 and 50 g/L were studied. The other treatment is the myo-inositol concentration, 100, 50, 10 and 5 mg/L were used. Based on the observation, MS media with 40 g/L sucrose and 10 mg/L myo-inositol showed the best growth of callus.

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