Reproductive biology study of Bornean red-eye puffer, Carinotetraodon borneensis

Syamira Najha, Binti Abd Rahman (2017) Reproductive biology study of Bornean red-eye puffer, Carinotetraodon borneensis. [Final Year Project Report] (Unpublished)

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Abstract

Carinotetraodon borneensis (Regan, 1903) is a small, fresh water fish that are indigenous in the Southern part of Batang Kerang river, Balai Ringin, Serian, Sarawak and known by the name red-eye puffer. This study investigates the reproductive biology and maturity stages of the fish. Males are bigger than females with mean body weight, mean total length and mean standard length of 6.33(±2.12)g, 6.05(±0.71)cm and 5.01(±0.61) while females are 4.26(±1.23)g, 5.21(±0.54)cm and 4.38(±0.42)cm. Gonad weight and liver weight indicate the GSI and HSI value through specified calculation. GSI value is higher in female than male in three studied months (GSI: female-2.45%, male-1.11%). HSI value show significant difference for male and female (HSI: female- 2.37%, male 1.77%). Size, color, and condition of the gonad indicate the maturity stages. The testes are in stage II and III in August’16, stage III and IV in October’16 and stage I and II in January’17. The ovaries are in stage IV and V, stage V, and stage III and IV along the months. H&E protocol were used to stain 5 µm gonad cell for histology study. Microscopic images show growth phase of the gonad which are Oogonia stage and early perinucleolar stage.

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