Acclimatization of Tissue Cultured Neolamarckia cadamba Plantlets to Ex Vitro Conditions

Norsyuhadah, Binti Rosman (2017) Acclimatization of Tissue Cultured Neolamarckia cadamba Plantlets to Ex Vitro Conditions. [Final Year Project Report] (Unpublished)

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Abstract

Neolamarckia cadamba belongs to Rubiaceae family which is a fast-growing timber species. It is widely used as traditional medicines and suitable for reforestation. Demand for Neolamarckia cadamba is expected to be increased along with the high demand of wood products (6.4 million m' in 2020). Micropropagation technique helps in mass production of many plants species including Neolamarckia cadamba. However, transplanting the plants to ex vitro conditions continue to be the major bottleneck of micropropagation technique. This study was aimed to establish the suitable method for acclimatization of Neolamarckia cadamba. The plantlets were subjected to different acclimatization approaches. There is a control and two different acclimatization approaches (Method A and Method B). Each method has 3 replicate with 3 plantlets for each replicate. A control is whereby the plantlets were planted without being covered by transparent plastic bag, was used as a comparison with Method A and Method B. In Method A and Method B, the plantlets were covered by transparent plastic bag. However, in Method B, the plantlets were directly supplemented with Gamborg B5 liquid medium without vitamin and sucrose. Percentage ofplant survival rate, plant height, plant fresh weight and leaflength were analyzed by using one way ANOVA and Duncan's Multiple Range Test in SPSS Version 22. The experimental design used was Randomized Complete Design (RCD). The results show that there was significant difference in percentage of plant survival rate, plant height, plant fresh weight and leaf length in Method A and Method B compared to controL Yet, there was no significant difference in percentage of plant survival rate in Method A (88.89%) and Method B (88.89%). Hence, it can be concluded that Method A is the suitable method to acclimatize Neolamarckia cadamba as it is more simple method compared to Method B.

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