The Size Correlation Between The Thickness of Stratum Spongiosium and Average Size of the Biggest Skin Gland of Male Rhacophorus pardalis at Kubah National Park

Nur Hassanah, Mohd Hassan (2017) The Size Correlation Between The Thickness of Stratum Spongiosium and Average Size of the Biggest Skin Gland of Male Rhacophorus pardalis at Kubah National Park. [Final Year Project Report] (Unpublished)

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Abstract

For centuries, frogs skin have been studied and proven to have its own significant value and contributions. There are two distinct layer in the dermis of a frog's skin, namely stratum spongiosium and stratum compactum. Stratum spongiosium comprised of loose connective tissue hence providing many space for skin glands to co-exist within one layer. However, the correlation and the interdependency between the thickness of stratum spongiosium (the first layer of the dermis) and the average size of the biggest skin gland in that very same layer has never been described and discussed, especially in Malaysia. Therefore, this study was done to look into the relationship between these two variables and to study the difference between the thickness of the spongiosium layer from the dorsal and ventral skin parts by using male Rhachophrus pardalis from Kubah National Park as the study subject. Histological technique namely skin fixation, embedding, sectioning and staining by using the Hemotoxylin and Eosin (H&E) method were applied and histological analysis was run and calculated by using ImageJ software. Statistical analysis using simple linear regression and independent t-test were carried out to validify the interdependency relationship between the spongiosium layer and the average size of the biggest skin gland and also the difference between the thickness of this particuar layer in both dorsal and ventral skin parts. The result showed a significant correlation between the thickness of the spongiosium layer and the average size of the biggest gland in both dorsal and ventral parts. Independent t-test analysis also proved that there is a significant difference between the spongiosium layer in the dorsal part and the ventral part where the average mean of the layer's thickness is slightly higher in ventral rather than the dorsal.

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