Preliminary Study on Sugar Recovery from Fresh and Wet Cladophora sp

Nur Azira Zuleikha, Ahmad Zailani (2017) Preliminary Study on Sugar Recovery from Fresh and Wet Cladophora sp. [Final Year Project Report] (Unpublished)

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Abstract

Cladophora sp. is the green filamentous macroalgae that can be found abundantly in all water bodies, especially in freshwater and marine. In this study, the starch and reducing sugar content from fresh and wet Cladophora sp. taken from their natural habitat and through the cultivation was analyzed. The cultivation of these algae was conducted in three different media such as Drain water as a control (DW), Filtered Tap Water (FTW) and Filtered Sago Effluent (FSE) for 10 days. This Cladophora was collected every 2 days for 10 days from their natural habitat and through the cultivation for analysis of starch content. From the starch analysis the high concentration of starch taken from the natural habitat was recorded on the day 8 at 0.49 g/L. The cultivated Cladophora sp. in three different media such as DW, FTW and FSE has shown that the high starch concentration was recorded on day 4, 12.5 g/L for DW and 13.46 g/L for FTW whereas the FSE shown the high starch concentration on day 2 at 16.3 g/L. Once the 10 days cultivation was done the total left Cladophora sp are used for the hydrolysis process to analyze total reducing sugar obtained from this algae. Different hydrolysis was conducted such as enzymatic hydrolysis and acid hydrolysis using 5% dilutes Sulfuric Acid H2SO4. Total reducing sugar that has been analyzed via Dinitrosalicyclic acid (DNS) show that the highest number of reducing sugar was recorded at 0.158 g/L from the Cladophora sp. cultivated in the FSE through the acid hydrolysis.

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