Fermentation of Different Carbohydrate Media by Wickerhamomyces anomalus for Ethanol Production

Mazlyana, Binti Mohd Zainul (2017) Fermentation of Different Carbohydrate Media by Wickerhamomyces anomalus for Ethanol Production. [Final Year Project Report] (Unpublished)

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Abstract

Humans have been using fossil fuel as source of energy for centuries. Unfortunately, the combustion of fossil fuel releases pollutants, causing air pollution and global warming. Therefore, biofuel such as bioethanol is now being produced to replace fossil fuel. This research aims to study the production of ethanol from different carbohydrate media as well as analyze the different amount of ethanol production from anaerobic batch fermentation of different carbohydrate media using Wickerhamomyces anomalus and Saccharomyces cerevisiae. Apart from that, this research also aims to observe the growth pattern of W. anomalus and S. cerevisiae in the different carbohydrate media. The different carbohydrate media used were glucose and xylose. Samples were collected at 0, 6, 12, 24, 36,48 and 72 h. The same methods were used for fermentation of xylose media and the experiment was repeated using S. cerevisiae. Lastly, the concentration of ethanol produced and composition of other fermentation products were analyzed upon the completion of fermentation via High Performance Liquid Chromatography (HPLC), while the growth pattern of cells were analyzed using cell count method. From this experiment, the fermentation of glucose media using S cerevisiae yield a higher amount of ethanol which is 27.3 g/L compared to W. anomalus (21.2 g/L).

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