Preliminary Molecular Work on Sea Turtles of Sarawak

Amirul Azwan, Bin Ainie. (2008) Preliminary Molecular Work on Sea Turtles of Sarawak. [Final Year Project Report] (Unpublished)

PDF (Please get the password by email to repository@unimas.my , or call ext: 082-583914/3933/ 3942) Amirul Azwan Bin Ainie (fulltext).pdf Restricted to Registered users only Download (3MB)

Abstract

According to IUCN Red List of Threatened Species, sea turtles are categorized as endangered species. In Sarawak, they are enlisted as totally protected species. Changes in climate, decline in food source, anthropogenic and natural disturbances are such of causes. This research highlighted the preliminary molecular work on Sarawak sea turtles. Molecular biology methods involved in this project were Total Genomic DNA extraction, Polymerase Chain Reaction (PCR), and PCR-RFLP processes. This initial work could possibly be a stepping stone for future Sarawak sea turtles advanced research particularly in phylogeography, population genetics, and consequently produce sustainable conservation and management. This study showed that CTAB protocol has productively extracted total genomic DNA from sea turtle tissue. An approximately 300-400 bps D-loop region were successfully amplified via PCR techniques and restriction enzymes of Alui, BamHI and Csp6I managed to give PCR-RFLP profile.

Actions (For repository members only: login required)

View Item