Rice Blast Disease Screening and Phytochemical Analysis of Local Rice

Cbia, Yi Zhen (2015) Rice Blast Disease Screening and Phytochemical Analysis of Local Rice. [Final Year Project Report] (Unpublished)

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Abstract

Rice (Oryza sativa) is a major food crop throughout the world. In Sarawak, there are at least 10 types of rice varieties available; however rice blast disease caused by Magnaporthe oryzae is still threatening to local rice production. Disease screening had found out that the seedling of Bario Halus is susceptible, while Biris and MR219 were resistance to local rice blast varieties Magnaporthe oryzae 2(1)'. The flavonoid profiling for eight local rice varieties had been done, intending to associate with the resistance of local rice varieties to rice blast. Unfortunately, establishing relation of local rice variety flavonoid profile to rice blast disease resistance was not as facile as expected due to low conidiation of local rice blast isolates that hampered the disease inoculation. A test of fungal growth rate was then conducted and the result suggests local rice blast pathogen in pH 5 and pH 7 OMA at 28°C grown best. The primers were also designed for four A vr genes while degenerate primers were design to two A vr genes.

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