Morphological identification and (GTG)_5-PCR fingerprinting of endophytic fungi isolated from akar gaharu

Lim, Chai Fen (2014) Morphological identification and (GTG)_5-PCR fingerprinting of endophytic fungi isolated from akar gaharu. [Final Year Project Report] (Unpublished)

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Abstract

Agarwood is a valuable incense produced from Aquilaria trees that are infected by fungi. The price of agarwood has increased dramatically throughout the years and its contribution to the economy is significant. However, the high demand of agarwood has exceeded its supply due to its high value and multiple functions. In order to overcome this problem, fungi can be used as an inoculant to induce agarwood formation. Akar gaharu is an aromatic tendril plant that produces inferior agarwood, which is also infected by fungi. In this study, the fungi species isolated from the akar gaharu were being compared to the ones commonly found in agarwood. The purposes of this project were to identify and group the fungi isolated from akar gaharu into different species. Morphological observation and molecular approaches which involve DNA extraction, PCR amplification and clustering analysis were used for characterization of the fungi. DNA fingerprinting method was carried out to group the fungi into different species. The identified fungi species were Trichoderma sp., Penicillium sp. Beauveria sp., Mucor sp. and Phoma sp. and they were grouped into two major clusters based on their genetic distance. Among the identified species, Trichoderma sp. and Penicillium sp. were considered to be related to agarwood formation. These fungal isolates can be utilized in formulating a microbial consortium to induce agarwood formation.

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