Characterization of expression pattern RPS3, BolA1 and MIF genes in normal and tumour human cell line

Liew, Joe Yee (2014) Characterization of expression pattern RPS3, BolA1 and MIF genes in normal and tumour human cell line. [Final Year Project Report] (Unpublished)

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Abstract

Protein-coding genes such as ribosomal protein S3 (RPS3), BoIA homolog 1 (BoZAJ) and macrophage migration inhibitory factor (MIF) are the genes that regulate protein synthesis, redox potential and immunological function respectively. Bioinformatics study has postulated the possible interaction of these proteins in silico and the possible association in carcinogenesis. Literature reviews have reported the up-regulation of RPS3 and MIF in different types of carcinoma cell lines. However, expression and interaction study involving these genes have not been conducted. The objectives of this study were to compare and characterize the expression patterns of these genes in human nasopharyngeal non-malignant cell line and nasopharyngeal malignant cell lines. Reverse transcription polymerase chain reaction (RT-PCR) had been employed to examine the expression level of gene transcripts. PCR products of RPS3 and MIF with the estimated size of 428bp and 221bp were targeted in this study, whereas expression of Bo1AI gene was undetectable due to unspecific primer. At the end of this study, RPS3 and MIF have manifested up-regulation in tumour cell lines as compared to the normal cell line however there was no significant difference between expression of RPS3 and MIF (p>0.05).

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