An attempt to detect avian encephalomyelitis virus among unvaccinated village chickens in Kuching division, Sarawak

Mohd Ruzaimi, Bin Rusli. (2007) An attempt to detect avian encephalomyelitis virus among unvaccinated village chickens in Kuching division, Sarawak. [Final Year Project Report] (Unpublished)

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Abstract

Five districts in Kuching Division, Sarawak namely, Bau, Batu Kawa, Siburan, Bako and Lundu were chosen and ten unvaccinated village chickens were randomly taken from villages in each of the district for AEV detection. Specific and sensitive reverse transeriptionpolymerase chain reaction (RT-peR) assay was used for the detection of avian encephalomyelitis virus (AEV) ribonucleic acid (RNA). 5'UTR peR primers were used to amplify the 5'untranslated region (UTR) of AEV genome. An AEV-specific 494 base pair cDNA product was amplified by these pimers from positive control but no products were amplified from all of the clinical samples. The results suggested that RT-peR provided a specific and sensitive approach for identification of AEV isolates.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2007.
Uncontrolled Keywords: Avian encephalomyelitis virus, village chickens, primers, reverse transcriptionpolymerase chain reaction, complimentary DNA, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 13 Sep 2019 06:57
Last Modified: 18 Sep 2023 07:09
URI: http://ir.unimas.my/id/eprint/26822

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