Extraction and Characterisation of Collagen from The Skin of Bluespotted Stingray (Dasyatidae kuhlii)

Mohamad Fais, Che Nuddin (2015) Extraction and Characterisation of Collagen from The Skin of Bluespotted Stingray (Dasyatidae kuhlii). [Final Year Project Report] (Unpublished)

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Abstract

Collagens were known for its function to replace the human collagen for sustainability of human health. The demand for the quality collagens in so many industry especially pharmaceuticals and cosmetics are extremely high. Acid soluble collagen (ASC) from the skin of bluespotted stingray, Dasyatidae kuhlii were extracted and characterised. The method developed by Nagai and Suzuki (2000a) with slightly modification were used for the extraction process. The yield of ASC was 7.3% based on the wet weight of the skin. The characterisations of the collagens were determined using Fourier Transform Infra Red and Scanning Electron Microscope. Biological evaluation was conducted using Brine Shrimp lethality test for cytotoxicity. The antioxidant activity, antibacterial and antifungal were also conducted. The results suggest that bluespotted stingray skin has potential as an alternative source of collagen for use in various fields.

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