Analysis of leaf Epicuticular wax in several Zingiberaceae species from Sarawak

Nur Sulwana, Binti Mohd Hanapi (2010) Analysis of leaf Epicuticular wax in several Zingiberaceae species from Sarawak. [Final Year Project Report] (Unpublished)

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Abstract

Several species belong to Zingiberaceae were collected from various locations in Sarawak. The leaf epicuticular waxes were extracted by using n-hexane and then analyzed with gas chromatography-mass spectrometry. Analysis of epicuticular waxes were carried out to determine the present and amount of n-alkanes. Subsequently, the concentrations of aliphatic hydrocarbon were determined. The total aliphatic hydrocarbons of these nine species ranging from 83.05 Ilglg to 802.71 Ilgig. In the Plagiostachys crocydocalyx and Etlingera elat;or, Cli was the most abundant n-alkane (32.74 Ilg/g and 41.22 Ilglg). In the Z. sp. A, A/pinia /atilabris and A/pinia aquatica, C29 was the highest concentration of aliphatic hydrocarbons with 37.86 Ilgig, 100.44 Ilgig and 134.42 /lg/g, respectively. A/pinia galanga, Etlingera inundata, Etlingera nasllta and Amomum xanthophyllum had high concentration at C25 (29.99 Ilgig), C23 (105.47 /lgig), C21 (52.59 /lgig) and C20 (14.29 Ilg/g). The most abundance n-alkanes in leaf epicuticular waxes are higher molecular weight alkane with odd-numbered carbon, particularly in range of C21 to C33 • The compound identified in the leaf epicuticular waxes was analyzed statistically using cluster analysis to determine their correlation between species. Cluster analysis performed showed a good separation among the species of Zingiberaccae in this study. The data obtained provided further evidence of the chemotaxonomic significance of leaf wax n-alkanes.

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