Vegetative Propagation of Papaya (Carica papaya L)

Anny, Jong (2007) Vegetative Propagation of Papaya (Carica papaya L). [Final Year Project Report] (Unpublished)

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Abstract

Papaya (C{[/"Ica papaya L.) is herbaceous perennial frUit crop of tropical and subtropica l countries. Majority of commercial papaya plantations prefer 100% hermaphrodIte papaya trees. Vegetative propagation of papa ya is the onl y method to produce papaya planting stock of the desired sex type . Papaya can be propagated clonally by graftIn g of selected scion s on eight months old trees. bH:ilm propagation IS another me thod of vegetallve propagation. Establishment of contamination-free cuhure lS the pre-requisite for I.n-\'ilro propagatIon . To reduce the level of con tamination. the shoot-tip explan ts should be taken from lhe newly sprouled or Oushed shools of prwled trees. Resull of th is siudy showed tha I high percentage of axenic cull ure from field·denved explanl could be achieved by subject the explant material to three rounds of spray wilh 3.75% neultralized phosphoric acidal weekl y Interval and followed by surface sterilization in 20% 'Clorox' for) 5 mlnutes, cultured on MS mecliunl incorporated With PPMnl at 3m IlL. For mductlOn of multiple shoot formation frOI11 the axenic shoot-tip explants, 0.25 mg/L of NAA and 0.5 mg/L of BAP were found to be effective. Furth ermore, MS medium was found to be more superior to the B5 medium in sequence uf shoot proliferation for seedling's shoot tips.

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