Effect of liquid mycelium culture on spawn production of oyster mushroom pleurotus sajor-caju (fr.) singer

Bong, Sik Chee. (2016) Effect of liquid mycelium culture on spawn production of oyster mushroom pleurotus sajor-caju (fr.) singer. [Final Year Project Report] (Unpublished)

PDF (Please get the password from Digital Collection Development Unit, ext: 082-583932 / 082-583914) Effect of liquid mycelium.pdf Restricted to Registered users only Download (1MB)

Abstract

Pleurotus sajor–caju (Fr.) Singer (grey oyster mushroom) is an edible mushroom which is a high temperature species in the group of Pleurotus (oyster) mushroom. The aim of this study is to determine the best liquid medium for the growth of P. sajor-caju mycelium and to investigate the effect of liquid mycelium culture on the spawn production of P. sajor–caju. The liquid inoculum had the ability to reduce the duration of the mycelium growth period in the paddy grain spawn media. Two types of culture media were prepared which were liquid and solid culture media. Four types of different liquid media were prepared which include basal media, honey, brown sugar and malt extract. The basal media of liquid media consisted of KH₂PO₄ (0.05 g), MgSO₄ ·7H₂O (0.05 g), FeSO₄ (0.01 g), KNO₃ (1.55 g) in 1000 mL of distilled water. Complex carbon compounds which are commercial honey and brown sugar were supplemented at the rate of 10 g/1000 cm³. 17g of malt extract was mix in 1000mL distilled water. Malt extract broth media was the best media for the P. sajor–caju mycelia to grow with the proven of highest dry weights of mycelia (0.067±0.0033g) collected compared to honey (0.043 0.0067g) and brown sugar (0.033±0.0033g) liquid media. Mycelia of malt extract broth inoculum also fully colonized the paddy grain spawn in the shortest period compared to mycelia of honey and brown sugar inoculum.

Actions (For repository members only: login required)

View Item