Comparative leaf anatomy of Tetrastigma species (Vitaceae) from Gunung Mulu National Park, Sarawak

Nur Aishah, binti Amir. (2016) Comparative leaf anatomy of Tetrastigma species (Vitaceae) from Gunung Mulu National Park, Sarawak. [Final Year Project Report] (Unpublished)

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Abstract

Tetrastigma is a genus from the Vitaceae family that consist about 122 species worldwide. This species can be found in subtropical regions of South East Asia and Australia. Study on morphology of Tetrastigma has been done extensively. However, anatomical study on this genus is still limited. Therefore, the objective of this study was to investigate the anatomical variations in leaves of Tetrastigma species from Gunung Mulu National Park in order to see if anatomical characteristics from leaves can be used to identify species. In this study, the specimens collected from Gunung Mulu National Park, Miri were used to observed anatomical characteristics of the midrib, lamina, and leaf margin. Sliding microtome was used in leaf sectioning and double-stained in Safranin and Alcian green. Then, the dehydration was done in an alcohol series. The section mounted in Euparal before left the section to dry in the oven at 60ºC. The sections were observed under the light microscope and images captured using a digital camera fitted on a microscope and processed using Motic Image Plus 2.0 software and saved as JPEG file. The anatomical characteristics that have been observed in this study are vascular bundles, outline of midrib as well as the presence of the druses and trichomes in theTetrastigma species.

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