Molecular study of psammobia rostratus (ambal goyang) from Buntal Bay, Sarawak

Mohd Fazhan, Bin Mohd Hanafiah (2009) Molecular study of psammobia rostratus (ambal goyang) from Buntal Bay, Sarawak. [Final Year Project Report] (Unpublished)

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Abstract

Pcannnohia rostratus (Antbai Govang) is one of the species of clams that spread along Buntal bay area. P. rostratus is filter feeders that plays important role in ecological services. They are harvested by local people from August to March during low tide either for domestic consumption or side income. The aims of this study were to amplify and sequence the 16S rRNA gene, COI gene, ITS gene and ITS1 gene, documented the PCR-RFLP restriction profile of ITS gene. The modified CTAB DNA extraction method and modified Phenol Chloroform DNA extraction method were used to extract the total genomics DNA of about 33 samples of P. rostratus from Buntal bay area. In this study, the amplification of the mitochondrial gene, 16S rRNA gene generated fragment size approximately 550 bp whereas the COI gene only generated fragment approximately 350 bp. For nuclear DNA gene, the size of ITS gene are approximately 1000 bp and no positive result yielded for the amplification of ITS I gene. After PCR, the 16S rRNA gene, COI gene and ITS gene were purified and sent to commercial company for sequencing but the sequencing result was noisy and cannot be used for further analysis. The PCR product of ITS gene were subjected for PCR-RFLP analysis using 10 restriction enzymes (REs) namely Csp61, Hindlll, Tagl, Mholl, BsuRI, MnII, MspI (Hpall), Mbol, Ail and Rsal. Excluding Csp6I and Hindlll, all REs successfully revealed the restriction profile.

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