Genetic diversity of the benthic dinoflagellate, ostreopsis ovata (dinophyceae

Law, Fanny Pei Ying. (2014) Genetic diversity of the benthic dinoflagellate, ostreopsis ovata (dinophyceae. [Final Year Project Report] (Unpublished)

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Abstract

Benthic dinoflagellates are important component in the coral reef ecosystems. Ostreopsis is receiving high attention as it has the ability to produce potent neurotoxins that are harmful to human. This study intends to investigate the genetic population structure of the benthic dinoflagellate, Ostreopsis cf. ovatu in Malaysian water by using the secondary structures of the second internal transcribed spacers transcripts (ITS2) of the nuclear encoded ribosomal RNA (rRNA). The morphology of Ostreopsis was observed under epifluorescence and SEM. A total of 10 strains of Ostreopsis spp from Borneo were cultured and genomic extracted. Nucleotide sequences obtained in this study was used as template to model the secondary structures. Free energy minimization and homologous modeling were applied for structural analysis. The structural information was incorporated into the multiple sequence-structure alignment for phylogenetic analysis. The phylogenetic analysis of O. ovata species have resulted in two main Glade which is Indo-Pacific Glade and Mediterranean-Atlantic Glade. Strain from Borneo water have hemi-CBC with strain from others locality (Port Dickson, Indonesia, Greece, Portugal, Brazil and Italy). However, they are no CBC or hemiCBC discovered between strains from Borneo water and Redang. The orthologous sequence-structure on profile neighbor joining has provided a better insight into the genetic diversity of O. ovata in Malaysian water.

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