Effects of castor oil on Escherichia coli K011 growth and activities during batch anaerobic fermentation

Nur Hanani, Binti Hailmy. (2014) Effects of castor oil on Escherichia coli K011 growth and activities during batch anaerobic fermentation. [Final Year Project Report] (Unpublished)

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Abstract

Escherichia coli KO 11 has been one of the organism of choice in the study of bioethanol production since it is one of the facultative anaerobes that has excellent growth and researchers are all familiar with its fermentation capabilities under anaerobic environment. In this study, the growth and activities of E. coli KO11 monitored along the process of batch anaerobic fermentation. Both of the carbon sources, xylose and glucose were consumed during fermentation. The addition of castor oil showed different results in the growth and fermenting activities of E. coli KO 11 because castor oil has been known to have the ability reduce the bioethanol concentration in the fermentation broth. The viable cell count and High Performance Liquid Chromatography (HPLC) results explained that the presence of castor oil affected the ethanol production and growth of E. coli K011 and the best growth were in 10% of glucose-xylose in the fermentation broth without castor oil. E. coli KO 11 has higher tendency to consume glucose first compared to xylose hence producing bioethanol but both of the carbon sources are favoured. When castor oil was present, the bioethanol concentration was lowered so it did not disrupt the enzymes for its metabolic activities. The highest ethanol production recorded was in 10% glucose-xylose in fermentation broth without castor oil, 47.77% of theoretical yield. Hence, either xylose or glucose alone was preferred to be consumed by E. coli KO 11 for a better production of bioethanol so that tendency factor can be omitted.

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