Ellavia, Juakim (2014) Eric-pcr analysis of escherichia coli isolated from small mammals and water samples. [Final Year Project Report] (Unpublished)
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Abstract
Escherichia coli are free living bacteria that mostly harbour the of mammals and water sources. E. coli is also a common foodbome bacteria that causes illness and deaths in human. The objective in this study is to determine the relatedness of six E. coli strains isolated from small mammals and water using Enterobacterial Repetitive lntcrgenic Consensus Polymerase Chain Reaction (ERICPCR). The analysis of the E. coli strains by ERIC-PCR produced DNA bands of different sizes for differentiation purposes and cluster analysis at similarity of 84.8% grouped the strains into 2 different clusters. Based on the dendogram generated, the most related isolates were E. coli that is isolated from small mammals, grouped within cluster A. The E. coli isolate from Hipposideros larvarus (Intermediate roundleaf bat) oral cavity were more closely related to E. coli 0 157:H7 reference culture, EDL933 with 99.6% similarity index. The ERIC-PCR that is adapted in this study was shown to possess high discriminatory power for determining the relatedness of E. coli strains isolated from small mammals and water
| Item Type: | Final Year Project Report |
|---|---|
| Additional Information: | Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2014. |
| Uncontrolled Keywords: | Escherichia coli, foodbome bacteria, Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR), relatedness, small mammals, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Karen Kornalius |
| Date Deposited: | 02 Aug 2018 07:22 |
| Last Modified: | 01 Aug 2023 09:28 |
| URI: | http://ir.unimas.my/id/eprint/21093 |
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