Lipid accumulation in lipomyces starkeyi MV-4 cultured in glucose media

Ummi Syahida, Binti Zamri. (2015) Lipid accumulation in lipomyces starkeyi MV-4 cultured in glucose media. [Final Year Project Report] (Unpublished)

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Abstract

Nowadays, biodiesel has gained a lot of attention and the demand for it also increased. The conventional method to produce biodiesel from plant oil has some limitations. It leads to the discovery of Lipomyces starkeyi MV-4 (L. starkeyi MV-4) that could accumulate lipid up to 70% of its dry biomass weight. The aim of this study was to increase the lipid produced when cultured in different concentration of glucose. Two different glucose concentrations that were used were 2.5% (w/v) and 5.0% (w/v). The optimum growth of L. starkeyi MV-4 has been identified on sixth day. The highest dry biomass weight and lipid produced when cultured in 2.5% (w/v) of glucose was at day eight with 17.23 g/L and 3.73 g/L respectively. When cultured in 5.0% (w/v) of glucose, the highest dry biomass and lipid accumulation was at day seven with 17.90g/L and 4.36g/L respectively. The phenol- sulphuric test was performed to determine the glucose profile. The residual of glucose for 2.5% (w/v) and 5.0% (w/v) of glucose were 0.06g/L and 0.29g/L respectively.

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