Identification of pseudomonas spp. isolated from wild animals in Saremas Plantation, Miri, Sarawak

Choo, Chee Wei. (2014) Identification of pseudomonas spp. isolated from wild animals in Saremas Plantation, Miri, Sarawak. [Final Year Project Report] (Unpublished)

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Abstract

Pseudomonas spp. are found to be prevalence in wild animals. Propagation of these opportunistic microorganisms among human and animals became important for public health concern. In this study, Pseudomonas spp. from the feacal, anal, intestine and mouth in wild animals in a conserved forest located at Saremas Plantation, Miri, Sarawak were identified using biochemical tests and detection of exotoxin A (ETA) gene. Series of conventional biochemical test were done, including gram staining, oxidase test, triple sugar iron test, citrate test, catalase test, macconkey agar test, urease test, growth at 42 ℃ and 4 ℃ and cetrimide agar test to identify the isolates based on enzymatic reactions and metabolic pathways. Through these tests, 12 out of 68 isolates were identified as Pseudomonas spp., including P. aeruginosa, P. chlororaphis, P . cichorii, P. fluorescen, P. putida and other Pseudomonas spp. Five isolates were subjected to detection of 369-bp gene, ETA by using PCR and nonspecific bands were observed. This study suggest that Pseudomonas spp. is prevalent in the mouth and anal sample of wild animals, including snake, birds, and bat

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