In vitro regeneration of shorea parvifolia dyer ssp. parvifolia

Kho, Pei Ee. (2005) In vitro regeneration of shorea parvifolia dyer ssp. parvifolia. [Final Year Project Report] (Unpublished)

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Abstract

ABSTRACT Shorea parvifolia Dyer ssp. parvifolia or meranti sarang punai is a woody species from Dipterocarpaceae family. It is economically important and its timber as well as nontimber products have long been recognized in Southeast Asian and other regions. The present study was carried out to establish the micropropagation protocols for use in largescale propagation and the development of an in vitro regeneration system for the use in genetic manipulation of S. parvifolia Dyer parvifolia. Lamina, internodal, nodal and shoot tips explants from healthy 2-year-old seedlings of S. parvifolia Dyer parvifolia were surface sterilized in 70% ethanol prior to treatment of 10% (v/v) clorox with I mIll Tween 20 for IS minutes. Internodal, nodal and shoot tip explants were inoculated on Y2 MS (without vitamins and sucrose) soft agar medium incorporated with IS mIll PPM, 10 mlll tetracycline and 3g/1 activated charcoal. Meanwhile lamina explants were inoculated on the same formulation of medium except reducing the PPM to 10mlll. After three days, the explants were subcultured to Y2 MS soft agar medium which incorporated with S mill PPM and S mlll tetracycline. BAP was found effective for shoot growth of this species. There was about 80% of shoot tip explants sprouted new shoots on Y, MS soft agar medium incorporated with Imlll PPM and BAP either at O.Smgll or 2.Smgll. Meanwhile, only SO% of nodal explants sprouted axillary buds on Y, MS soft agar medium supplemented with lmlll PPM and O.Smgll BAP. Most of the nodal explants failed to react with 1.0mgli and 2.Smgll BAP. Yellowish and greenish granulated callus were successfully induced from cut edges of internodal explants cultured on Y, MS soft agar medium supplemented with I mill PPM, Kinetin at 0.2mgll along with O.2mg/l NAA. However, lamina and internodal explants failed to induce callus formation on Y, MS soft agar medium supplemented with different concentration of2, 4-D, and picioram.

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