Comparative molecular analysis og re site patterns among members of the human p53 gene family

Wong, Sook Hooi. (2006) Comparative molecular analysis og re site patterns among members of the human p53 gene family. [Final Year Project Report] (Unpublished)

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Abstract

Thep53 gene family consists of three family members -p53,p63 andp73. Thep53 gene is an established tumour suppressor gene and functions in growth an-est, apoptosis, DNA synthesis and repair, genome stability and cell differentiation. The p63 and p73 genes are involved in growth, development and differentiation, whereby the p63 gene is required for limb fon-nation and epidellnis fon-nati on whereas the p73 gene is essential for neurogenesis and pheromone signalling. In this study, p53-gene family members were amplified with RT-PCR using total RNA from bladder tissues. The purifIed RT -PCR products were then subjected to restriction digestion using Haelll, Hin 6I, Hpall, MboI, Pstl, RsaI, SmaJ, TaqI and Tru9I to obtain the RE site patterns for each gene family member. These pat1ems were analyzed and compared for variation assessment. The PCR-RFLP analysis yielded no observable results. Simulation of restriction digestion using MB Advanced DNA Analysis version 6.8 1 so fiwar'e showed that theoretically, the p53 and p73 genes showed higher similarity to each other as compared to with p63 gene, consistent with previous studies.

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