Isolation of cDNA fragment encoding isoamylase gene from metroxylon sagu by rt-pcr method

Rohana, Binti Musa. (2006) Isolation of cDNA fragment encoding isoamylase gene from metroxylon sagu by rt-pcr method. [Final Year Project Report] (Unpublished)

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Abstract

Isoamylase is one of starch debranching enzymes (DBEs) that involves in starch biosynthesis. The study of isoamylase gene from Metroxylon sagu (sago palm), which considered very productive in starch production, could provide a better insight in understanding the gene properties and its function. Isoamylase gene have been characterized in maize, rice, potato, barley and wheat in previous study but it is yet to be explain in sago palm. For identifying isoamylase gene in sago palm, total RNA was extracted and RT-PCR technique was performed. cDNA was synthesized and simultaneously amplified by RT-PCR metbod, which offers rapid, most sensitive and versatile way in analyzing gene expression. Isoamylase degenerated primers were design for priming specific gene in PCR reaction. Nevertheless, in this study, the isolation of fragment encoding isoamylase gene in sago palm was not achieved as the full length ofexpected isoamylase gene fragment was not obtained.

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