Anticancer activity screening of selected chalcone compounds towards caov-3 cell line

Chew,, Pey Ling. (2011) Anticancer activity screening of selected chalcone compounds towards caov-3 cell line. [Final Year Project Report] (Unpublished)

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Abstract

Chalcone compounds have been known to possess anticancer activity. The present study is a preliminary investigation on the anticancer potential of eight newly-synthesized chalcone compounds towards CaoV-3 cell line. Vero cell line, which was commonly used in the laboratory, was used as a standard. Eosin B staining assay method revealed that the compounds CI and C2 possessed high cytotoxic effect towards cancer cells following a 48-hour incubation period. The anticancer activity ofCI at 25 )lglml and 100 Ilglml, and C2 at 50 )lglml and 100 Ilglml did not differ significantly from the positive control at p=0.05. This suggests that the compounds were as effective as the positive control in killing the cells at these concentrations. C7 has also been shown to be a good candidate for developing into an anticancer drug, since the compound was mildly cytotoxic to Vero cells at 72hour incubation period and showed a large difference in cytotoxicity between 50 Ilglml and 100 Ilglml, which implie that controlled dosage of C7 can be used to regulate its activity. Structure-activity relationship (SAR) analy is showed that the intensity of anticancer activity was influenced by the location of substituent group (in ring A or ring B), their positions within the aromatic ring, and the chain length of hydrocarbon substituents. In addilion, RT-PCR was performed to detect the expression level of Cox-2 gene in order to evaluate the capability of the compounds in suppressing Cox-2 gene expression. However, no Cox-2 band was detected in all of the samples studied. One postulation for this observation was the defect in the primers used. Nonetheless, further test..... such as DNA sequencing or the use of different sets of Cox-2 primers, need to be carried out to confirm the postulation.

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