Evaluation of existing storage buffer for quality containment of dna extracted from animal cell line

Jayasutha, D/O Kubidasamy. (2010) Evaluation of existing storage buffer for quality containment of dna extracted from animal cell line. [Final Year Project Report] (Unpublished)

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Abstract

Deoxyribonucleotide acid (DNA) is one of the vital materials for studies in molecular biology. Good preservation of this material in proper storage buffers can maintain the integrity and quality of the DNA. This study focuses mainly on the quality of DNA stored in different storage buffers. The quality of DNA was determined by the degree of degradation in variety of buffers under a different storage condition. Our main objective was to find out whether or not the DNA stored in different storage buffer has different effect on the DNA. The DNA extracted from nasopharyngeal carcinoma (HKI) cell lines was used to facilitate this study. The extracted DNA was subjected to agarose gel electrophoresis (AGE) for qualitative analysis and Ultraviolet (UV) spectrophotometer for quantitative analysis. Three different storage buffers which are Tris-EDT A (TE) buffer, Tris-EDT A-NaCl (TES) buffer and nuclease free water was used under different storage period (ranging from 1 week to 1 month) and different temperatures (from -80°C to room temperature). The study concluded that nuclease free water is the most suitable storage buffer to store DNA at any temperatures for any storage period. It also states that TE buffer is the most unstable buffer that causes variation in DNA purity reading.

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