PCR optimization and validation of species-specific scar marker derived from damd in Eusideroxylon zwageri Teijsm & Binn

Lee, Jolly Shiat Mei. (2006) PCR optimization and validation of species-specific scar marker derived from damd in Eusideroxylon zwageri Teijsm & Binn. [Final Year Project Report] (Unpublished)

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Abstract

The aim of this tudy was to develop species-specific molecular markers for Ellsideroxylon Zl-vageri to allow its proper identification, in order to effectively conserve this valuable timber species. E. zwageri or more well-known as Belian, the Borneo Ironwood is one of the most durable timber species which is endennic to the Borneo island and its population i decreasing extensively due to over exploitation and de-forestation. Thus, conservation on the species is important. However, E. nvageri is morphologically and anatomically similar to another species of belian known as Potoxylon melagangai thus causing problems in differentiating these two species. Hence, Sequence Characterized Amplified Region (SCAR) primer set was designed using Primer Premier™ 5 software. This SCAR marker amplified specific band only from E. zwageri. The size of expected band is 719 bp. PCR optimization was carried out to fmd the right reagent conditions and thennal cycling profiles for the SCAR primer set. Validation of the SCAR marker was done using eight E. zwageri samples. Sample of P. melagangai is also included to ensure there is no incident of false positive. It is noted that the validation confmned that only E. zwageri samples produce the specific band and the fragment contained no obvious repetitive sequence beyond the primer. Thus, it is concluded that SCAR marker is potentially useful to easily identify the E. zwageri in a population.

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