Chung, James Yaw Ming. (2005) Restriction site mapping analysis of the adenomatous poliposis coli (APC) gene in human colorectal system. [Final Year Project Report] (Unpublished)
![[img]](http://ir.unimas.my/style/images/fileicons/text.png) |
PDF (Please get the password by email to repository@unimas.my , or call ext: 3914 / 3942 / 3933)
Restriction site mapping analysis of the Adenomatous poliposis coli (APC) gene in human colorectal system (fulltext).pdf Restricted to Registered users only Download (14MB) |
Abstract
Adenomatous Poliposis Coli (APC) anomalies are considered to playa gate-keeping role in colorectal tumorigenesis. Even though APC gene is extensively studied, there is a lack in effort towards creating a highly reproducible and less costly approach to diagnose APC anomalies. In this research, the APC cDNA fragments from the normal and tumour colorectal specimens are subjected to mutational analysis via restriction enzyme site characterization. Restriction digestions using AluI, HaeIII, Hinfl, HincH, HpaII, MboI, NdeI, RsaI, TaqI, and Trn9I were performed on all eight APC fragments of normal and tumour specimens accordingly. Restriction site map for the gene was constructed whereby assay on APC 2, APC 5, and APC 6 had generated differential restriction site pattern. Tumour APC 2 was showing aberrancy on RsaI, Hinfl, Trn9I, HaeIII, and HpaH; whereas tumour APC 5 was aberrant on AluI, and tumour APC 6 was aberrant on TaqI. This will facilitate the recognition of potential mutation marker to screen for APC aberrancy, allowing the comparative study between APC restriction site pattern of normal and tumour system.
| Item Type: | Final Year Project Report |
|---|---|
| Additional Information: | Project report (B.Sc.) - Universiti Malaysia Sarawak, 2005. |
| Uncontrolled Keywords: | Adenomatous Poliposis Coli (APC) gene, tumour suppressor gene, restriction enzyme (RE) pattern, exon 15, Molecular genetics Research Methodology, Genetic engineering Research Methodology, Genetic Techniques, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Saman |
| Date Deposited: | 12 Oct 2017 06:50 |
| Last Modified: | 13 Feb 2023 08:28 |
| URI: | http://ir.unimas.my/id/eprint/18074 |
Actions (For repository members only: login required)
 |
View Item |
