Screening of alcohol dehydrogenase genes in Sago palm

Enizza, Kasim. (2006) Screening of alcohol dehydrogenase genes in Sago palm. [Final Year Project Report] (Unpublished)

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Abstract

Plants usually respond to the environmental changes that can be caused by abiotic factors such as low oxygen concentration by changing their physiology and morphology adaptation. Plants inhabiting in a flood prone area or poor drained soil condition are subjected to severe growth condition. Thus, understanding how alcohol dehydrogenase (Adh) genes confer waterlogged tolerant species such as sago palm to endure the harsh conditions during an anaerobic stress is crucial in order to manipulate them in the nearest future. Therefore, the study was carried out to identify and clone the Adh genes of sago palm before sequencing can be done. DNA from sago palm was successfully extracted using the CT AB method whereby fine bands were observed after agarose gel electrophoresis had been done. The DNA was subjected for Polymerase Chain Reaction (peR) using three different primer sets, by which bright bands were produced for MorAdh primer set while haAdh-FI haAdh-R2 primer set, faint multiple bands were obtained. The fragments were gel excised and cloned into E.coli JM 109. Plasmids of white colonies were isolated prior to PCR amplification. However, the inserted of DNA fragments were failed to be detected. One PCR fragment was sent for direct sequencing and satisfactory result was obtained.

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