Isolation of cdna fragment encoding starch synthase gene from metroxylon sagu by rt-pcr method

Farizan, Bin Nan. (2006) Isolation of cdna fragment encoding starch synthase gene from metroxylon sagu by rt-pcr method. [Final Year Project Report] (Unpublished)

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Abstract

Metroxylon sagu have a big potential to compete with wheat, barley, rice and corn as food supply for human population in this region. Our understanding of starch biosynthesis and chemistry in most of important food supplier plant has advanced rapidly over the last few years, but our knowledge for starch synthesis in Metroxylon sagu is still lacking. In order to under stand the biochemistry of starch in Metroxylon sagu, genes that control starch biosynthesis in sago palm have to be known first. Degenerated primer for targeting a specific gene of starch synthase in Metroxylon sagu have been design using other plant starch synthase sequences that are closely related to Metroxylon sagu. It is found that this primer could amplify targeted gene at temperature ranging between 40°C to 48°C. but this primer does not amplified the full length of starch synthase gene. An expected fragment targeted by this primer was 1.2 kb in length but the cDNA fragment that was isolated has a size of 100 bp to 200 bp in length.

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