Rossi, Shannan L. and Nasar, F. and Cardosa, Mary Jane (2012) Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains. Virology Journal, 423. pp. 58-67. ISSN 1743-422X
PDF
Genetic.pdf Download (111kB) |
Abstract
Four serotypes of dengue virus (DENV 1–4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100 million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation of both the 5′- and 3′-untranslated regions but considerable divergence within the open reading frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2, where human strains produced a larger number of progeny than sylvatic strains in human liver cells but not in monkey or mosquito cells.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | Dengue virus (DENV), Sylvatic DENV, Human DENV, Phylogenetic and phenotypic analyses, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak |
Subjects: | Q Science > Q Science (General) |
Divisions: | Academic Faculties, Institutes and Centres > Institute of Health and Community Medicine Faculties, Institutes, Centres > Institute of Health and Community Medicine |
Depositing User: | Karen Kornalius |
Date Deposited: | 20 Sep 2017 07:56 |
Last Modified: | 24 Jan 2022 06:44 |
URI: | http://ir.unimas.my/id/eprint/17689 |
Actions (For repository members only: login required)
View Item |