Viability of cucumis melo L. embryos following dehydration technique

Dianasih, binti Yaman. (2004) Viability of cucumis melo L. embryos following dehydration technique. [Final Year Project Report] (Unpublished)

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Abstract

A study was done to estimate the effectiveness of dehydration technique in cryopreserving Cucumis melo L. embryos. Dehydration methods used were laminar flow, with sucrose and silica gel. In laminar flow, the embryos were exposed under the laminar for 0, 2, 4, 6, 8 and 10 hours with the air flow of0.46 meters per second. Another technique was that the embryos were immersed in sucrose solution at 0.2, 0.4, 0.6, 0.8 and 1.0 M at 0, 15, 30, 45, 60 and 75 minutes. For silica gel, the embryos were left in desiccators for 0, 24, 48, 72, 96 and 120 hours. The initial test showed that the moisture content, viability and germination of the embryos used were 5.8%, 90% and 70% respectively. From regression analysis, the method that gave the highest duration of storing was by using sucrose at 0.2 M for 45 minutes. The viability was 90% and the embryos could be kept in liquid nitrogen for four months and five days.

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