Production of bacterial pectinase using banana peel agricultural waste as substrate

Amsal, Binti Abdul Ghani. (2009) Production of bacterial pectinase using banana peel agricultural waste as substrate. [Final Year Project Report] (Unpublished)

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Abstract

Bacterial pectinases have emerged as important industrial enzymes with wide-ranging applications recently. III this study, two bacterial strains obtained from orange peels indicated their ability to produce pectinase fiom qualitative and quantitative screening using iodine-potassium iodide and ONS assay method, respectively. Morphological, biochemical and molecular characteristics using 16S rONA analysis were done 10 identify the both strains and their identities are continned to be Bacillus subtilis and Bacillus sp. cp-h24. Production of pectinase from both bacteria strains using banana peels agricultural waste as substrate under aubmerged fermentation has been studied. Higher polygalacturonase activity produced by Bacillus subtilis (13.03 U/m1) and Bacillus sp. cp-h24 (21.18 Vlml) confinned that banana peels can be a better replacement to commercial pectin (9.785 Vlml and 14.14 Vlml, respectively). Optimization studies of the growth medium a wed that Bacillus subtilis produced higher polygalacturonase activity with 10% wlv banana peels (109.8 UIml). at 24 hours fermentation (52.61 Vlml), pH 10 (33.55 Vlml) and temperature 37°C (53.38 Vlml) while IIDciIlus sp. cp-h24 produced higher polygalacturonase activity with 5% wlv banana peels (91 .28 Vlml), at 24 hours fermentation (52.33 Vlml), pH 7 (38.27 Vlml) and temperature 37°C (49.66 Vlml). The isolation of pectinase-producing bacteria from agricultural waste and also the use of agricultural waste to produce maximum pectinase suggest strategic ways to reduce the production cost in enzymes commercialization.

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