Octavio, Carvajal-Zarrabal and Cirilo, Nolasco-Hipolito and Kopli, Bujang and Ayaaki, Ishizaki (2009) Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch. Journal of Industrial Microbiology & Biotechnology, 36 (3). pp. 409-415. ISSN 1476-5535 (online)
|
PDF
Production of nisin Z using Lactococcus lactis (abstract).pdf Download (122kB) | Preview |
Abstract
A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. This resulted in a volumetric productivity of nisin Z, as 50,000 IU l-1 h-1 using a cell concentration of 15 g l-1, 30 � C, pH 5.5 and a dilution rate of 1.24 h-1. Adding 10 g l-1 YE and 2 g l-1 polypeptone, other inducers were unnecessary to maintain production of nisin. The operating conditions of the reactor removed nisin and lactate, thus minimizing their effects which allowed the maintenance of cells in continuous exponential growth phase mode with high metabolic activity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Nisin Z, Bacteriocin, Sago starch, Membrane bioreactor, Lactococcus lactis IO-1, research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education |
| Subjects: | Q Science > QR Microbiology S Agriculture > S Agriculture (General) |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Engineering Faculties, Institutes, Centres > Faculty of Engineering |
| Depositing User: | Karen Kornalius |
| Date Deposited: | 17 May 2017 07:26 |
| Last Modified: | 17 May 2017 07:26 |
| URI: | http://ir.unimas.my/id/eprint/16285 |
Actions (For repository members only: login required)
 |
View Item |
