Phytochemical and biological studies on cinnamomum griffithii

Husna, A. Hamid (2006) Phytochemical and biological studies on cinnamomum griffithii. [Final Year Project Report] (Unpublished)

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Abstract

The stem barks of Cinnamomunr grif/ithii was extracted using methanol, filtered and evaporated to dryness to give 552.84 g (23.03 % weight / dry weight) crude extract. Solvent partition was performed using solvent with increasing polarities and resulted in four partitions which are hexane (8.12 g), dichloromethane (0.76 g), ethyl acetate (5.91 g) and methanol partition (10.4 g). Further purification of hexane partition and ethyl acetate partition were resulted in one pure compound (greenish crystal from H27 combined fraction) and four semi pure compounds (isolated from H 15,1-11 5b, H 16, E2 and E5b). The pure compound showed the R, value of 0.8 in chloroform-ethyl acetate (3: 2) and exhibited the molecular mass of 256 g/mol corresponded to molecular formula of C 151-112Ow4 ith melting point of 195.0°C 195.8"CC. Infrared spectra showed an absorbance bands at 3390 cm-1 (014 group), 1629 cm-1 (C=0 group), 1603cm_1 (C=C aromatic) and 1300 cm-' (presence of C-0). Based on information from the mass spectrum, infrared, melting point and comparison with published data, the pure compound has been identified as pinocembrin. Meanwhile, the semi pure compounds were identified as 1,2 -dimethoxy-4-(2-propenyl)-benzene, 3-phenylmethyl-2-propanoic acid, 5-ethyl-3,5-dimethyloxazolidione and 1,2-benzenedicarboxylic acid. Toxicity test against larvae of Artemia salincr was performed and hexane partition showed inhibitory activity against the larvae of Artemia salina with LC5� value of 82.54 pg/ml.

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