Kho, P.E. and Hamsawi, Sani and Boyce, P.C and Lim, S.L (2010) In Vitro Propagation of Globba brachyanthera K. Schum. Asia-Pacific Journal of Molecular Biology and Biotechnology, 18 (1). pp. 19-22. ISSN 0128-7451
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In Vitro Propagation of Globba brachyanthera K.Schum (abstract).pdf Download (123kB) | Preview |
Abstract
An in vitro propagation system was developed for Globba brachyanthera K.Schum., a potential ornamental plant by surface sterilized adventitious bulbils in 20% Clorox for 20 minutes and cultured on Gamborg B5 medium supplemented with a biocide Plant Preservative Mixture (PPM) and antibiotic tetracycline. Shoot tips (2-5mm) obtained from in vitro cultured plantlets were induced to form shoots on Gamborg B5 medium containing 20% sucrose and 2.8 g/L Gelrite supplemented with various concentrations of 6-benzylaminopurine (BAP) ranging from 1.0 to 3.0mg/L, either individually or in combination with alpha-naphthalene acetic acid (NAA) at 0.1 mg/L or 0.5 mg/L. All treatments induced formation of multiple shoots as well as rooting after 8 weeks of culture. The highest multiplication rate of 6.6 shoots per explants was obtained in Gamborg B5 medium supplemented with 3.0 mg/L BAP. The generated shoots elongated on Gamborg B5 medium and the multiplication rate did not change further in all of the successive subcultures.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Globba micropropagation, Direct organogenesis, Ornamental ginger, research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education |
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Academic Faculties, Institutes and Centres > Institute of Biodiversity and Environmental Conservation Faculties, Institutes, Centres > Institute of Biodiversity and Environmental Conservation |
| Depositing User: | Karen Kornalius |
| Date Deposited: | 22 Nov 2016 04:40 |
| Last Modified: | 22 Nov 2016 04:40 |
| URI: | http://ir.unimas.my/id/eprint/14391 |
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