Hasnain, Hussain and Chong, Nikson Fatt Ming (2016) Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis. BioMed Research International, 2016. ISSN 2314-6133
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Abstract
The combined overlap extension PCR (COE-PCR) method developed in this work combines the strengths of the overlap extension PCR (OE-PCR) method with the speed and ease of the asymmetrical overlap extension (AOE-PCR) method. This combined method allows up to 6 base pairs to be mutated at a time and requires a total of 40- 45 PCR cycles. A total of eight mutagenesis experiments were successfully carried out, with each experiment mutating between two to six base pairs. Up to four adjacent codons were changed in a single experiment. This method is especially useful for codon optimization, where doublet or triplet rare codons can be changed using a single mutagenic primer-set, in a single experiment.
| Item Type: | Article |
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| Uncontrolled Keywords: | combined overlap extension PCR method, Site Directed Mutagenesis technique, Mutagenesis, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Karen Kornalius |
| Date Deposited: | 16 Nov 2016 02:24 |
| Last Modified: | 17 Feb 2017 02:43 |
| URI: | http://ir.unimas.my/id/eprint/14294 |
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