Genetic variation in several population of macrobrachium rosenbergii de man deduced from sequencing of cytochrome c oxidase I (COI) mitochondrial DNA gene

Mat Nahi, Zuriani (2005) Genetic variation in several population of macrobrachium rosenbergii de man deduced from sequencing of cytochrome c oxidase I (COI) mitochondrial DNA gene. [Final Year Project Report]

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Abstract

This study examines the genetic differentiation of Macrobrachium rosenbergii in several populations in Sarawak. The analyses were carried out using the 154 base pair Cytochrome c oxidase I (COl) mtDNA gene. The samples used in this study were collected from four sites in Sarawak; Sungai Samarahan, Batang Rajang, Batang Lupar and Sungai Roban. Phylogenetic analysis was done using two methods, Neighbor-joining and Maximum Parsimony. The result from phylogenetic analysis shows the existence of two subclusters of udang galah; one cluster consisted of samples from Sarikei, while another cluster consisted of samples from Sg.Roban, Btg.Lupar and Sg.Samarahan. However, further studies with higher number of samples from other population and longer sequences were needed to verify the results of this study. In general, this study indicated the potential of using COl mtDNA gene in detection of even small genetic variation, or synonymous substitution between samples.

Item Type: Final Year Project Report
Additional Information: Project report (B.Sc.) -- Universiti Malaysia Sarawak, 2005.
Uncontrolled Keywords: Macrobrachium rosenbergii, genetic variation, Cytochrome c oxidase I (COl) mtDNA, DNA sequencing, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 21 Mar 2014 02:10
Last Modified: 29 Nov 2017 01:33
URI: http://ir.unimas.my/id/eprint/1369

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