Biodegradation of Lignin In Oil Palm Empty Fruit Bunch (EFB) Using Igninolytic Fungi.

Siong,, Teck Kwong (2009) Biodegradation of Lignin In Oil Palm Empty Fruit Bunch (EFB) Using Igninolytic Fungi. [Final Year Project Report] (Unpublished)

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Abstract

The research was carried out to investigate the degree of lignin degradation on oil palm empty fruit bunch (EFB) and secretion of Iigninolytic enzymes by Cerrena sp under varying cultures conditions mainly on different incubation periods, pH values, substrate types and combination of different activators. The solid substrate fennentation was conducted. Lignin content loss in EFB was quantified using modified Klason method. Spectrophotometric enzyme assays were conducted to detect extracellular ligninase and cellulase. Protein concentration was measured using Bradford method. Result revealed that the optimum cultures conditions were at day 12, pH 6, with the combination of MgS04+CaCb as activators. Un crushed EFB have been found be an ideal substrate for solid state fermentation. Cerrena sp yields the highest lignin peroxidase on day 12 with 2.423 U/m! of enzyme been produced. However, the highest laccase was on day 15 with 1.069 U/m! oflaccase been produced. The amount of the secretion of cellulose by the white rot fungi was measured, ranging from 0.085 U/m! to 0.l87 U/m!.

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