Noninvasive genetic sampling on the rare sumatran rhinoceros (Dicerorhinus sumatrensis): Identification of the host species from the blood meal collected from the tabanid fly (Tabanidae: Haematopota sp.)

Jeffrine Japning, Rovie-Ryan and Zainal Zahari, Zainuddin and Wahap, Marni and Abdul Hamid, Ahmad and Laurentius N., Ambu and Junaidi, Payne (2013) Noninvasive genetic sampling on the rare sumatran rhinoceros (Dicerorhinus sumatrensis): Identification of the host species from the blood meal collected from the tabanid fly (Tabanidae: Haematopota sp.). Asian Pacific Journal of Tropical Biomedicine, 2 (12). pp. 930-934.

Preview

PDF noninvasive genetic sampling (abstract).pdf Download (203kB) | Preview

Abstract

Objective:To demonstrate a noninvasive large mammalian genetic sampling method using blood meal obtained from a tabanid fly. Methods:Blood meal was recovered from the abdomen of an engorged tabanid fly(Haemotopota sp.) which was captured immediately after biting a Sumatran rhino in captivity.the blood was applied on to a Whatman FTA blood card. Subsequent laboratory work was conducted to extract,amplify and sequence the DNA from the sample. Validation was done by sampling the hair follicles and blood samples from the rhinocheros and subjecting it to the same laboratory process. Results:BLAST search and constructed phylogenetic trees confirmed the blood meal samples were indeed from rhino. Conclusions: This method could be used in the field application to noninvasively collect genetics samples. Collection of tabanids and other haematophagous artropods(e.g.mosquitoes and ticks) and other blood-sucking parasites (e.g. leeches and worms) could also provide information on vector-borne diseases.

Actions (For repository members only: login required)

View Item