Isolation and cloning of ABCA2 gene from rasbora sarawakensis

Tan, Say Yen. (2015) Isolation and cloning of ABCA2 gene from rasbora sarawakensis. [Final Year Project Report] (Unpublished)

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Abstract

ABC genes encode ABC transporter protein that plays an important role in ATP hydrolysis in which to assist the solute to cross the plasma membrane. Mutation of ABC genes lead to human diseases such as Alzheimer, PFIC, and PXE. The aim of this research is to identify the expression of ABCA2 gene in Rasbora sarawakensis and subsequently clone into pGEM-T® Easy vector. Total RNA was initially isolated from whole fish homogenate using Tri reagent and phenol chloroform precipitation.

Item Type:	Final Year Project Report
Additional Information:	Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords:	unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak, ABC transporter, ABCA2, PCR, Rasbora sarawakensis.
Subjects:	R Medicine > RS Pharmacy and materia medica S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions:	Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User:	Karen Kornalius
Date Deposited:	21 Mar 2016 06:15
Last Modified:	01 Apr 2024 07:43
URI:	http://ir.unimas.my/id/eprint/11027

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