Sing, Tung Teng (2015) Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA. Fisheries Science, 2015 (81). pp. 831-838. ISSN 1444-2906
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NO 99 Pseudo‑nitzschia species (Bacillariophyceae) identification (abstract).pdf Download (411kB) | Preview |
Abstract
An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrialencoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed in silico and used to infer the molecular phylogeny of Pseudo-nitzschia. The primer pair was tested using genomic DNAs isolated from six species of Pseudo-nitzschia from Malaysia. The phylogenetic inference of cox1 was then compared to the LSU rDNA phylogeny. Phylogenetic reconstructions of both data sets revealed monophyly of Pseudo-nitzschia species complexes. The range of genetic divergences among Pseudo-nitzschia species were higher in the cox1 data set (3.5–20.4 %) compared to the LSU rDNA data set (0.1– 8.8 %). The present study suggests that high genetic divergence in cox1 of Pseudo-nitzschia species could be a useful genetic marker for DNA bar coding.
| Item Type: | Article |
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| Uncontrolled Keywords: | cox1, Diatom, LSU rDNA, Primer, Pseudo-nitzschia, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling |
| Divisions: | Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology Faculties, Institutes, Centres > Faculty of Resource Science and Technology |
| Depositing User: | Karen Kornalius |
| Date Deposited: | 01 Mar 2016 07:19 |
| Last Modified: | 21 Oct 2016 06:42 |
| URI: | http://ir.unimas.my/id/eprint/10719 |
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