Molecular Technique Identification Of The Microbial Population In Palm Oil Mill Effluent (Pome)

Wei, Christy Chan Sien and Lau, Seng and Awang Ahmad Sallehin, Awang Husaini and Azham, Zulkharnain and Kasing, Apun and Leslie, Bilung and Micky, Vincent (2015) Molecular Technique Identification Of The Microbial Population In Palm Oil Mill Effluent (Pome). Journal of Oil Palm Research, 27 (3). pp. 293-298. ISSN 1511-2780

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Abstract

Bio­con­ver­sion of palm oil mill efflu­ent (POME) to gen­er­ate methane gas via anaer­o­bic diges­tion involves a con­sor­tium of microbes which are respon­si­ble in sev­eral steps of the biodegra­da­tion process. In this study, the micro­bial com­mu­nity from a selected POME was char­ac­terised via mol­e­c­u­lar tech­niques and through culture-based plat­ing in order to deter­mine their com­po­si­tion, and sub­se­quently under­stand their func­tion in the anaer­o­bic com­mu­nity. Genomic DNA of the micro­bial com­mu­nity was extracted using direct extrac­tion tech­nique, fol­lowed by PCR tar­get­ing the 16S rDNA region. Dis­tinct frag­ments of approx­i­mately 1100 bp in sizes were suc­cess­fully ampli­fied using PCR and cloned onto Escherichia coli XL-1 Blue. Upon sequenc­ing of the frag­ments, BLAST queries iden­ti­fied the bac­te­ria as Ther­moanaer­obac­terium sp. In addi­tion, two other bac­te­r­ial species were suc­cess­fully iso­lated from the POME by cul­tur­ing on DVS agar. The sequenc­ing result of these bac­te­r­ial iso­lates showed both iso­lates belonged to the Bacil­lus genus. By under­stand­ing the bac­te­r­ial com­mu­nity present in the POME, this will lead to the improve­ment of the anaer­o­bic diges­tion process to enhance the pro­duc­tion of bio­gas such as methane.

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