Screening and identification of microbes with potential antagonistic activity against phytophthora capsici

Tang, Lok Ing (2015) Screening and identification of microbes with potential antagonistic activity against phytophthora capsici. [Final Year Project Report] (Unpublished)

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Abstract

Phytophthora capsid infection on the black pepper can affect the production of black pepper. This infection can be managed using fungicides such as metalaxyl, copper oxychloride and phosphonate. However, the use of these fungicides against the infection can cause damage to the environment. Thus, it is important to look for a new approach to control P. capsid. The aims of this study are to isolate and characterize the bacteria that are antagonism against P. capsid. In this study, the bacteria were screened for their antagonistic activity through spore germination test and double plate assay. Hence, KDKBB83 and SB (2)6 that showed high antagonistic activity was selected for molecular characterization. The mo lecular characterization was done by using 16S rRNA sequence analysis. Analysis of 16S rRNA sequencing suggested the closest identities of the bacterial isolates as Serratia marcescens and Pseudomonas aeruginosa by using Basic Local Alignment Search Tool (BLAST) via National Center for Biotechnology Information (NCBI).

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