Establishment of axenic culture and callus induction of synsepalum dulcificum daniel (miracle berry)

Tan, Hong Wee (2015) Establishment of axenic culture and callus induction of synsepalum dulcificum daniel (miracle berry). [Final Year Project Report] (Unpublished)

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Abstract

Synsepalum dulcificum is a slow growing shrub from family Sapotaceae. The fruits contain high medicinal values which aid in chronic diseases due to the presence of active component Miraculin, a taste modifying properties. The current research emphasizes on the axenic cultures establishment and callus induction of Synsepalum dulcificum from leaf and seed explants. The sterilized leaf explants were obtained from both 25% Clorox® solution (Sodium hypochlorite) which produced 91.7% axenic cultures and 25% CaOCI solution (Calcium hypochlorite) that produced 94.4% axenic cultures with 20 minutes exposure time upon addition of 1% Benomyl solution respectively. The sterilized leaf explants were cultured onto Murashige & Skoog (MS) media supplemented with various concentrations of 2,4- Dichlorophenoxyacetic Acid (2,4-D), and Picloram. No callus was induced despite some of the leaf explants remained viable after certain period of time and most of the leaf explants results in browning conditions whereas callus was induced from seeds supplemented with 2.0 mg/L of 2,4-D after 3 weeks of culturing. The poor callus induction from leaves was highly due to the presence of high poly-phenolic compounds within the miracle berry plants. Future work should utilize different PGR‟s at different concentrations and also the use of different explants.

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